Peptide reference · GHRH analog with maleimidopropionyl-lysine (DAC)
CJC-1295 (with DAC) COA check: molecular weight, formula and what to look for
Use these reference values to check the identity result on a CJC-1295 (with DAC) Certificate of Analysis. The theoretical average molecular weight of CJC-1295 (with DAC) is 3,647.19 Da and the monoisotopic mass is 3,645.015 Da.
Check a CJC-1295 (with DAC) COA nowCJC-1295 (with DAC) reference data
| Name | CJC-1295 (with DAC) |
|---|---|
| Also known as | CJC-1295 DAC, DAC:GRF |
| Type | GHRH analog with maleimidopropionyl-lysine (DAC) |
| Molecular formula | C165H269N47O46 |
| Average molecular weight | 3,647.19 g/mol (Da) |
| Monoisotopic mass | 3,645.0155 Da |
| Residues | 30 |
| Reference source | PubChem CID 91971820 (formula and mass cross-checked) |
Expected mass spectrometry ions
A CJC-1295 (with DAC) COA may list one of these m/z values instead of the neutral mass. That is still consistent with CJC-1295 (with DAC). How charge states work.
| Ion | m/z (monoisotopic) | m/z (average) |
|---|---|---|
| [M+3H]3+ | 1,216.01 | 1,216.74 |
| [M+4H]4+ | 912.26 | 912.80 |
| [M+5H]5+ | 730.01 | 730.45 |
| [M+6H]6+ | 608.51 | 608.87 |
What else can show up on a CJC-1295 (with DAC) COA
Masses calculated from CJC-1295 (with DAC)'s formula for the adducts, degradation products and synthesis impurities a lab report can show. A match tells you what the extra peak probably is.
| Signal | Value | What it means |
|---|---|---|
| Na adduct, deconvoluted (M + 21.98) | 3,667.00 Da | Sodium adduct: an MS artifact, not a separate impurity. The [M+Na]+ ion itself is at M + 22.99 |
| K adduct, deconvoluted (M + 37.96) | 3,682.97 Da | Potassium adduct: an MS artifact. The [M+K]+ ion itself is at M + 38.96 |
| TFA adduct (M + 113.99) | 3,759.01 Da | Trifluoroacetic acid from purification seen as an adduct; residual TFA salt is real vial content |
Adduct rows are deconvoluted masses (the shift seen against [M+H]+); the singly charged [M+Na]+ ion itself sits at M + 22.99. Deletion masses alone can't say which copy of a repeated residue is missing.
At over 3 kDa, CJC-1295 (with DAC) is harder to purify; 98% or higher with one sharp main peak is a strong result. The full checklist is in how to read a peptide COA.
CJC-1295 (with DAC)-specific things to watch
CJC-1295 with DAC adds a lysine carrying a maleimidopropionyl group (the "Drug Affinity Complex") to the Mod GRF backbone, bringing the mass to about 3647.2 Da.
If a with-DAC product's COA shows a mass near 3368 Da, it is actually the no-DAC version. That is one of the most common labeling errors in the research peptide market, and this checker flags it automatically.
The maleimide group is reactive, so storage and age matter. A recent test date is more important for this compound than for many simple linear peptides.
Compounds with similar mass
If a COA's mass is off, compare it with these neighbors. A match to one of them can point to a mislabeled product.
- CJC-1295 (no DAC): 3,367.90 Da (-279.29 Da)
- Sermorelin: 3,357.88 Da (-289.31 Da)
- Semaglutide: 4,113.58 Da (+466.39 Da)
Sources
- PubChem CID 91971820: CJC-1295 (with DAC) formula and reference mass.
- CIAAW: Standard atomic weights: Used to calculate average molecular weights.
- NIST CODATA: proton mass: Used to convert m/z charge states to neutral mass.
- PeptideCOACheck methodology: How the checker's thresholds work.
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Frequently asked questions
What is the molecular weight of CJC-1295 (with DAC)?
The average molecular weight of CJC-1295 (with DAC) (C165H269N47O46) is 3,647.19 Da and its monoisotopic mass is 3,645.0155 Da, calculated from the molecular formula and cross-checked against PubChem CID 91971820.
What other masses can show up on a CJC-1295 (with DAC) COA?
Besides the main peak, a CJC-1295 (with DAC) COA can show a sodium adduct (deconvoluted mass near 3,667.00 Da). The table on this page lists every expected variant with its mass.
For laboratory research use only. This page describes analytical chemistry data. It is not medical advice and makes no claim about effects in humans or animals.